Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS) flower were investigated. The 100% methanolic crude extract showed the highest total phenolic content (40.33 mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. extract that inhibits the formation of DPPH radical by 50% (Moyo et al., 2013; Ndhlala et al., 2013).
Antioxidant and Cytotoxic Activities of 20160131 7671 1uznr9p With Cover Page v2 - Free download as PDF File (.pdf), Text File (.txt) or read online for free. the antioxidant plant extract is added to the . Antioxidant activity was based on the absorbance on the final day of FTC method. 22 It was reported that DPPH free radical scavenging activity of water extract of barberry (2 mg mL-1) was 74.08%. Solanum elaeagnifolium is among the invasive plants of Morocco; studies on its chemical composition and biological activities are few in number in Morocco. The radical scavenging activity of the FRAP activity of combined plants extract was higher as compared to their individual effect; the trend did not hold in the case of DPPH-radical scavenging activity.
The substances can be divided in three main groups , as depicted in Table 1. The DPPH radical scavenging activity is generally quantified in terms of inhibition percentage of the pre-formed free radical by antioxidants, and the EC 50 (concentration required to obtain a 50% antioxidant effect) is a typically employed parameter to express the antioxidant capacity and to . . Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth .
The aim of our study was to investigate the antioxidant properties of five plant extracts by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method, cupric reducing antiox-idant capacity . The free radical scavenging (DPPH) and the radical cation decolorization (ABTS) assays were conducted as described by Ballesteros et al. The IC 50 values were calculated from graphs by plotting extract concentrations vs. percentage inhibition carried out in triplicate and the averages of the three values were Antioxidant activity in traditionally used plant species is a method of scientific validation of the medicinal plant use by Indigenous Peoples. The results are highly reproducible and comparable to other free radical scavenging methods such as ABTS (Gil, Tomas-Barberan, Hess-Pierce, Holcroft, & Kader, 2000 The antioxidant activity is expressed in terms of IC 50
A lower value of IC 50 represents higher antioxidant activity. The plant extract showed remarkable antioxidant activity. 2.3.
The DPPH through the IC 50 values showed that fruits (12.16 g/ml) and seeds (11.41 g/ml) of Z. lotus from Bengardane and Oued Esseder were endowed with an interesting antioxidant activity. The oven-dried DS flower was extracted using 100% methanol (w/v), 100% ethanol (w/v), and 100% water (w/v). This difference may be due to the extract . Report. However, P. argentea exhibited the lowest amount of TPC (83mg GA/g dry extract). Scribd is the world's largest social reading and publishing site. (DPPH) assay is the most commonly used antioxidant assay for plant extract.
Antioxidant activity3.2.1. in this group, the highest values were observed for aacidS (95.7%) followed by acidG, VitE and SodasG (p>0.05). Results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter, and show the best discrimination of differences and/or similarities between species is considered. Formulation of Poly Herbal Hair Oil . The low antioxidant activity could scavenging (58.23-84.91% DPPH ) and antioxidant support the fact that the high antioxidant activity of properties (149.87-273.28 mol AAE/g DM) (Figures the aqueous decoction was the result of a synergistic 5 & 6). In vitro cytotoxicity and antioxidant activity of . At 5 mg/g, the extract was most effective indicating that higher concentration of extract gave higher an-tioxidant activity.
Phytochemical Screening and in vitro Antioxidant Activity of Jawarish Amla- A Poly Herbal Formulation . L-Ascorbic acid was used as the reference compound. Antioxidant activity: 3.3.1. The DPPH assay is a typical off-line detection method, where the antioxidant activity is measured colorimetrically. .
The effectiveness of the extract was determined using DPPH at 50 mg/g, 10 mg/g and 5 mg/g of the extracts. 552 nm. [ 8] isolated extracts from leaves, fruit pulp, and seed of R. idaeus and identified their polyphenols by HR-HPLC-ESI-qTOF-MS/MS method.
The antioxidant activity differences observed among different species of Ziziphus may be attributed to phenolic compounds, which depend on the region [23 . All the R. idaeus' extracts, especially those from leaves, exhibited . DPPH assay is a reliable method to determine the antioxidant capacity of biological substrates.
PROXIMATE AND PHYTOCHEMICAL ANALYSIS OF STEVIA LEAVES POWDER .
0 downloads 0 Views 807KB Size. Several methods have been developed to assess the radical scavenging activity.
The result of antioxidant activity of ethyl acetate extract Antioxidant activity test toward ethyl acetate extracts are done by measuring the absorbance of ethyl acetate extract for each concentration variation. DPPH stable free radical method is a sensitive way to determine the antioxidant activity of plant extracts (Koleva et al., 2002; Suresh et al . The objective of this research is to improve this plant and assess its antibacterial . Methanolic extracts of 13 commercially available citrus spp., peels and tissues growing in Iran were investigated for their antioxidant activity by DPPH method. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to . Fifty micro liters of the plant extract in methanol, yielding 100g/ml respectively in The 100% methanolic crude extract showed the highest total phenolic content (40.33 mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. To determine antioxidant activity 2,2-diphenyl-1-picryl-hydrezyl (DPPH) wasusedasfreeradical.100M concentration of DPPH wasusedinmethanol.Serialdilutionsweremadetocheck the IC50. Among them, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric method is one of the most widely applied and is appreciated for its reliability. Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl) assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II) chelating ability, and a ferric reducing antioxidant power (FRAP) assay.Total phenolic and flavonoid contents were determined using the Folin . ageratumconyzoides.pdf accessed on March, 2012. The DPPH method is described as a simple, rapid and convenient method inde-pendant of sample polarity for screening of many samples for radical scavenging activity (Marxen et al., 2007). the antioxidant activity of foods and beverages (Prakesh, 2001). Keywords
S. elaeagnifolium has shown molluscicidal and nematicidal and cancer-inhibitory effects, anti-inflammatory, analgesic activity, and antibacterial activity. Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical.
4 The DPPH assay method is based on . determined by the DPPH method among the extracts sub- . Download PDF . The objective of this research is to improve this plant and assess its antibacterial . From the absorbance measurement, IC 50 value of ethyl acetate extract is 36.18 mg/L.
Studies Analytical Chemistry, Pharmacognosy and Phytochemistry, and Materials Science. Wu et al. 11. .
The results from the antioxidant assay showed that extract of all plants can scavenge the radical to a certain extent. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method Luciana L. Mensor , Departamento de Produtos Naturais e Alimentos, Faculdade de Farmcia, Universidade Federal do Rio de Janeiro, Centro de Cincias de Sade, Bl. An examination of Table 4 reveals that the total antioxidant activity, measured by DPPH method, ranged from 0.20 to 1.50 mg trolox equivalent per g dry weight (mg, TEAC/g dw). Antioxidant Activity of Ethanolic Extract from Tandui Leaves (Mangifera rufocostata Kosterm.) The cytotoxic activity was evaluated in MCF-7, MCF-10A and HT-29 cell lines. Aliquots of 1 mL of each sample in the methanolic extract were collected (at 4 different concentrations: 0.1, 0.5, 1, and 2 mg/mL; two replicates per sample and concentration) and . (2020) assessed the antioxidant activity of extracts from A. domesticus in depth (DPPH, ABTS, FRAP, and FIC). It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds (Cook and Samman, 1996). In vitro antioxidant activity tests DPPH radical method In evaluating the free-radical-scavenging activity of crude extracts and single compounds, the agent of choice is often the DPPH radical. The aim of this study was to screen various solvent extracts of whole plant of Torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations.. Material and methods. [16,17] Here, the radical scaveng-ing activities of the crude ME of 14 different plants were assayed by measuring the decrease in absorbance of
The methanol extract and aqueous extract of the plant were tested for antioxidant activity using scavenging activity of (1,1-diphenyl-2-picrylhydrazil) radical method .The the methanolic extract exhibited high free radical scavenging activity when compared to aqueous extract.
A detailed study was performed on the antioxidant activity . From: Food .
IC50 was found to be 17 (g/ml).
plant species which showed that alcoholic extract of leaves of this plant on higher concentration possess better antioxidant potential when compare to reference standard ascorbic acid . A volume of 100 L of vary- 70 % ethanolic extract of P. daemia: The antioxidant activity of ing concentrations of the extract (5, 2.5, 1.25, 0.625, 0.3125, 0.1563, 70 % ethanolic extract of P. daemia (EEPD) was evaluated using 0.0781 and 0.0391 mg/mL) was added to 100 L of 2 % AlCl3, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical . An examination of Table 4 reveals that the total antioxidant activity, measured by DPPH method, ranged from 0.20 to 1.50 mg trolox equivalent per g dry weight (mg, TEAC/g dw). 1-picrylhydrazyl (DPPH). IC 50 for antioxidant activity ranged from 0.6-3.8 mg ml-1. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. After the DPPH was fully dissolved, the flask was filled up to the mark with methanol. Download Free PDF Download PDF Download Free PDF View PDF. substances exhibited antioxidant activity, except for cL. In this work, water extracts from different bio-based products of plant origin were studied to evaluate their antioxidant capacity and their potential to form metal nanoparticles from aqueous solutions. A recent stu dy has . , FRAP, H2O2 and DPPH assays. Studies for the determination of the antioxidant activity . The antioxidant activity of the In this study Antioxidant activity was performed by DPPH (1, 1diphenyl-2-picryl hydrazyl) radical scavenging method for different extracts of aerial parts like leaves and . In 96-well micro plate total volume was 100l which was consisting of 90l of DPPH solution and 10l Two traditional tests, the Folin-Ciocalteu assay and the DPPH radical scavenging capacity method were compared with a more recent . Methods. to evaluate antioxidant activity. The hydro-ethanolic extracts were obtained by matrix solid-phase dispersion (MSPD) and . From the methodological point of view the DPPH method is recommended as easy and accurate with regard to measuring the antioxidant activity of fruit and vegetable juices or extracts. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method . The radical scavenging activity of plant extracts against stable DPPH radical (DPPH*) was determined. A mass of 20 mg of DPPH was dissolved with a small amount of methanol in a 500 mL volumetric flask.